Ratetraene-



United States Patent A -ESTRATETRAENE-16;8,17,8-DIOLS AND 160: LOWER HYDROCARBON DERIVATIVES THEREOF Albert Bowers and Pierre Crabbe, Mexico City, Mexico,

assignors, by mesne assignments, to Syntex Corporation, a corporation of Panama No Drawing. Filed Mar. 1, 1962, Ser. No. 176,825

15 Claims. (Cl. 260-3975) The present invention relates to novel cyclopentanophenanthrene derivatives and to a process for the production thereof.

More particularly the present invention relates (to novel A -estratetraene-165,17B-diols and to novel 16a-lower hydrocarbon derivatives thereof.

The novel compounds of the present invention are rep- I,

' the '3-acylates thereof, is treated with isoamyl nitrite in resented by the following formula:

i 19 2 Patented Sept. 10, l963 ice . 'O R OH In the above formulas R, R R and R have the same meaning as hereinbefore set forth.

In accordance with the above equation the starting compound (1) which is selected from the group consisting of A -dehydro estrone, the 3-lower alkyl ethers and the presence of potassium t-butoxide for a period of time of the order of 6 hours at room temperature and thereafter with aqueous glycine and hydrochloric acid to produce the corresponding 16-oximino-A -dehydro estrone derivative (11). The latter lo-oximino compound upon In the above formula R represents hydrogen, lower alkyl or a hydrocarbon carboxylic acyl group of less than 12 carbon atoms; R and R represent hydrogen or a hy- 'drocarbon carboxylic acyl group of less than 12 carbon may be saturated or unsaturated, of straight, branched,

cyclic or cyclic-aliphatic chain, aromatic and may be substituted by functional groups such as hydroxy, alkoxy containing up to 5 carbon atoms, aoyloxy containing up to 12 carbon atoms, nitro, amino or halogen. Typical ester groups are the acetate, propionate, enanthate, benzoate, trimethylacetate, t-butylacetate, phenoxyacetate, cyclopentylpropionate, 'aminoacetate and [B-chloropropionate.

The compounds represented by the above formula have low estrogenic activity but are very valuable in arresting calcium excretion in certain bone conditons and catabolic states, such \as osteoporosis. In addition they lower the blood and adrenal cholesterol levels, are valuable in the treatment of arteriosclerosis.

. The novel compounds of the present invention are prepared by the process exemplified as follows:

y wh

reaction with zinc in aqueous acetic acid at reflux temperature for a period of time in the order of 1 hour, furnishes the corresponding A estratetraen-l7fiol-l6-one (Ill).

The 16-ketones II I) are treated with a lower alkyl, lower alkenyl or lower alkynyl magnesium halide as for example methyl magnesium bromide, vinyl magnesium bromide or 'ethynyl magnesium bromide in a solvent inert to the reagents, to produce the corresponding 16oz (lower alkyl, lower alkenyl or lower alkynyl-A -estratetraene-lomlm-diol derivatives ('IV: R =R =H).

The l7fl-hydroxy-il6 ketones H1) are reduced, preferably with sodium borohydride, in order to produce the corresponding 165,17B-diols (IIV: R =R =R H).

The secondary hydroxyls of the compounds set forth hereinbefore, such as the 17,8-hydroxyl group and/ or the 16B-hydroxyl group of the ,lfiwunsubstituted compounds, are conventionally acylated in pyridine with an acylating agent, as for example acetic anhydride or propionic anhydride, to give the corresponding 17 8 and/ or l6fi-acylates (*IV: R ='H, R=R =acyl). In the case of the 16w substituted compounds, this type of conventional esterification affords selectively the l7 3-acylates (IV: R =acyl,

' R =hydrogen, R i=lower hydrocarbon).

The 16B-tertiary hydroxyl group of the l6a-substituted derivatives (IV: R =H, R =lower hydrocarbon) is conventionally acylated in the presence of p-toluenesulfonic acidwith an acylating agent such as an anhydride derived from a hydrocarbon oarboxylic acid of the type described hereinbefore, to give the corresponding l6/3-acylate (IV: R =acyl, R =lower hydrocarbon).

The following specific examples serve to illustrate but are not intended to limit the scope of the invention:

Example I tanol there were added 2.5 g. of A -dehydroestrone-3- methyl-ether (Magerlein et al., I. Am. Chem. Soc. 80,

I 2220, [1958]). The resulting mixture was gently warmed until solution of the steroid was complete, then it was allowed to cool to room temperature. There were added a 1.8 cc. of isoamylnitrite with stirring, which was thereafter continued for 6 hours. The reaction mixture was allowed to stand at room temperature overnight. Then there were added 500 cc. of aqueous glycine followed by 7 cc. of concentrated hydrochloric acid. The mixture was extracted with 500 cc. of ether. The organic layer was washed once with 360 cc. of 3% aqueous sodium bicarbonate solution and then extracted with 500 cc. of 5% aqueous potassium hydroxide solution. Acidification of the basic aqueous phase with concentrated hydrochloric acid yielded a precipitate which upon recrystallization from methanol afiorded pure 16-oximino-A -dehydroestrone-3-methylether.

Example II 2.5 gfof the latter product were mixed with 2.5 g. of zinc dust and 125 cc. of 50% aqueous acetic acid and refluxed for 1 hour at the end of which the mixture was filtered through celite. The filtrate was concentrated to a small volume under reduced pressure, cooled and diluted with ice water to precipitate a crude product. Recrystallization from acetone-hexane afforded B-methoxy- M -estratetraeml713-01-16-one.

Example III 4 Example VII Example VIII A -estratetnaen-lZu old6-one was treated in accordance with Example V, thus yielding 16a-ethinyl- A -estratetraene-B,165,17B-triol.

Example IX A solution of 1 g. of sodium borohydride in 3 cc. of waiter was added to an ice-cooled solution of 1 g. of 3 meth0xy-A -estratetraene-17fi-ol-16-one, in 120 cc. of methanol and the mixture was allowed to stand for 16 hours at room temperature. The excess reagent was decomposed by addition of acetic acid, the solution concentrated to small volume in vacuo and diluted with water. The product was extracted with ethyl acetate, the extract was washed with water, dried and evaporated. The solid residue was purified by crystallization from acetone-hexane to give S-methoxy-A estratetraene-l6,8,175-di0l.

By the same techniquewas treated A -estratetraene-3,l7B-diol-l6-one, thus giving A -estratetraene-S, 1 6 6, 17fi-triol.

Example X A mixture of 1 g. of 3-methoxy-A -estratetraene-16fl,17 8-dio1, 4 cc. of pyridine and 2 cc. of acetic anhydride was kept at room temperature overnight, poured into ice water, the formed precipitate was filtered, washed with water and dried. Crystallization from me tone-hexane gave the diacetate of S-methoxy-A estratetraene-16B, 17fi-diol.

In the same manner were treated the starting compounds under I, thus yielding the corresponding products under H.

l7-acetate of 3-methoxy-16a-methyl-A M -estratetraene-l65,17B-dio1.

3,17-diacetate of lfia-methyl-A J 500) "-estratetraene-Eidfifld7fi-triol. ,17-d acetate of lfia-vinyl-A 3 M10) -estratetraene-3,16,8,17fl-triol. 8,17-d1acetate of lfiu-ethinyl-A 3 M10) -estratetraene-Ii,16,6,17B-triol.

Example IV Example V 3-methoxy-A i -estratetraen-l7fi-ol-16-one was treated in accordance with Example III, with the exception that ethinyl magnesium bromide was used instead of methyl magnesium bromide, thus giving 3-methoxyl6a-ethinyl-A -eStratetraene-I65,17 3-diol.

Example VI A -estrone (Magerlein et al., v. supra) was treated in accordance with Examples I, Hand III, giving successively: 16-oximino-A -estrone, A -estratetraene-3,I7fl-diol-16-one and 16a-methylA -es-- tratetraene-3, 1 6,8, 17fl-triol.

Example XI Upon treatment of the starting compounds of the foregoing example by the procedure described in. that example, but using instead of acetic anhydride, propionic anhydride, caproic anhydride and ,cyclopentylpropionic anhydride, there were produced the corresponding propionates caproates and cyclopentylpropionates of said starting compounds.

Example XII To a solution of 5 g. of 3-meth0xy-16a-methyl- A -estratetraene-16,6,17fl-diol in cc. of anhydrous benzene there were added 1 g. of p-toluenesulfonic acid and 10 cc. of propionic anhydride and the mixture was allowed to stand for 24 hours at room temperature, poured into ice and water, and the resulting mixture stirred to eifect hydrolysis of the excess anhydride. Ihe benzene layer was separated and washed with 10% sodium carbonate solution and water. Drying, evaporation and crystallization of the residue from etherhexane produced the dipropionate of 3-methoxy-16amethyl-A -estratetraene-16B,l7 8-diol.

By the same procedure were treated the starting compounds under A affording the corresponding products wherein R is selected from the group consis ting under B. hydrogen, lower alkyl and a hydrocarbon carboxythc acyl group of less than 12 carbon atoms; R and R are A B members of the group consisting of hydrogen and a 5 hydrocarbon carboxylic acyl group of less than 12 car- 3.methoxy-16a-viny1-A The dipropionate of 3-methoxybon alums R3 is Selected fil'om the group cows/5mg estmtemenelfilm-(111- g' 'fi'iggxn of hydrogen, lower alkyl, lower alkenyl and lower e raene- 1O a-mithtoxy-lfiu-etgny- 1- (11)- Th? difiiropifnat e ogfimethoxyy x es ra etraene-l ,17 i 1 we iny-A ,6 1 11 r 1,3,5(10),9(11) r estmtetmenedfifldwmol' 2. 3 methoxy 16oz methyl A estratetraene lfia-methyl-A -estra- The tripropionate of lfia-methyl- 10 I J tetraene-3,168,175-triol. 3 1%;ggg t piestratetraene- 3 3 h 16 i 1 1,3,5(l0).9(11) estratetraene- T10 16a-Viny1A -estratetraene- The tripropionate oi 16a-viny1- 165,1713-(1101. I

3,168,17/3-t1i0l. 1 [(n)iestratetraene- 4 vsflfneflqoxyfll6wefl1yny1 1,3,5(),9(11) t t t 1'10 16a-ethiny1-A ,9 -estra- The tripropionate of lfia-ethinyl- 16,8,175-(1101.

tetraene-3,16fi,17B-trio1. ink-2:1 9]???iestratetraene- 15 5 3 mh 1,3,5(10),9(11) q t t -16 3 17 -d i 1 r10 6. 16a-mefohy1-A estratetraene 3,165,175-

triol. Example 7. 16a vinyI-A estratetnaene 3,165,175-

The starting compounds of the foregoing example t i l, were treated by the procedure disclosed in that example, 20 8. 16u-ethyny1 A estnatetraene-3,16B,17B-

except that propionic anhydride was substituted by acetic trio-1.

anhydride and oaproic anhydm'de, thus afiording re- 9. M -estratetraene-3,16,8,17fl-triol. spectively the corresponding acetates and caproates. 10. The 3,17-diacetate of 16a-metihyl-A Example XIV estratetraene-3,165,17/3-triol.

11. The 3,17-diacetate of 16a-vinyl-A -es- Ihe 17-acetate of 3-methoxy-16a-methyl-A g w 17, m 1

estratetraene-16;8,17,8-diol was treated tfollowing the 12 Th 3,17-di t t ()[E 16 -eflhyny1-A technique described in Example XII, thus yielding the g gg 17 i 1 16 propionate 17 acetate of 3-methoxy-16u-methy1- 13 h ,triacetate f 1 1 1,s,5(10). UIL f fi fitetraene-3,16B,17B-triol.

We clalm: 14. The tri acetate of 16a-viny1-A -estra- 1. A compound of the following formula: tetraene-3,16fi,17B-tri0l.

15. The triacetate of 16u-ethynyl-A -estratetraene-3,1618,l7fl-trio1. H Ra References Cited in the file of this patent Fishman et a1., Jour. Org. Chem, vol. 2 3, 958, pages 1190-92. 

1. A COMPOUND OF THE FOLLOWING FORMULA: 